Composite
Part:BBa_K2092008:Design
Designed by: Hui Wen Chong Group: iGEM16_Manchester (2016-10-02)
Pcon (J23110) + RBS (B0034) + alcR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2611
Illegal BamHI site found at 1615
Illegal BamHI site found at 2353 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1319
Illegal AgeI site found at 1447
Illegal AgeI site found at 1978 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Calculated the RBS strength by using the online tool SalisLab RBS Calculator.
Source
Aspergillus nidulans. The protein was first extracted and characterized by <a href="https://www.ncbi.nlm.nih.gov/protein/AAQ06627.">Felenbok et al. (1988)</a> [1]. This version is codon optimized for E.coli by Thermo Fisher Scientific and synthesized by IDT.